Nā huahana
Bucladesine
Hui Pū ʻIa
Na Waiwai Kino
ʻAno: Huahana keʻokeʻo paʻa paʻa
Kūlana mālama: -20°C
Hiki ke hoʻoheheʻe H2O: 50 mg/mL
ʻIkepili Palekana
No ka waiwai ma'amau
Palapala noi
ʻO Dibutyryl cyclic adenosine monophosphate sodium he mea ia o ka cyclic adenosine monophosphate, i hoʻohana ʻia e mālama i ka haʻalulu cardiogenic a me ka pau ʻole o ka naʻau.I ka makahiki 1999, ua hoʻomohala ʻia ka ʻaila ʻaila Dibutyryl cyclic adenosine monophosphate sodium e mālama i ka maʻi ʻili a me ka maʻi ʻili.
Hoʻomākaukau o ka sodium dibutyryl cyclophosphate adenosine: I loko o ka ipu i loaʻa i ka cyclophosphate adenosine lohi e hoʻohui i ka wai sodium hydroxide solution (3 mol / L) dropwise, ke kaʻina hana i hoʻomaluʻia ka mahana, dropwise hoʻopau i ka mahana wela, hoʻololi i ka pH i 7 ~ 8 me ka HCl solution ( 20%), hoʻohui anhydrous ethanol precipitation o keʻokeʻo paʻa, kānana, maloʻo e loaʻa i ka paʻakai sodium paʻa keʻokeʻo;ka paʻakai sodium a me ka n-butyl anhydride ma lalo o ka pale o ka hoʻomehana ʻana o N2 i 140 ℃ pane.ʻO ka nānā ʻana o TLC, pau ka hopena a laila hoʻohaʻahaʻa ʻia i ka lumi wela, e hoʻohui mālie i ka methyl tert-butyl ether, ke kaʻina hana o ka hoʻoulu ʻana ikaika, Chemicalbook e loaʻa i kahi huahana paʻa paʻa keʻokeʻo.Ua hoʻokuʻu hou ʻia kahi hui ʻana o ka methyl tert-butyl ether a me ka acetone no ka loaʻa ʻana o kahi huahana paʻa keʻokeʻo.ʻO ka hana olaolaDibutyryl-cAMP(Bucladesine,dbcAMP) he mea hoʻoulu PKA cell-permeable e hana ana ma ka hoʻolike ʻana i ka cAMP endogenous. ʻO Dibutyryl-cAMP(Bucladesine) kekahi mea hoʻopaneʻe phosphodiesterase (PDE).
Nā haʻawina in vitro: Hoʻopaʻa ʻo Dibutyryl-cAMP i ka lawe ʻana i ka glucose neuronal ma o ka hoʻoulu ʻana o PKA.I loko o nā hepatocytes ʻiole moʻomeheu, Dibutyryl-cAMP kaohi inducible nitric oxide synthase hōʻike a me ka hana hoʻopaʻa NF-kappaB.ʻO ka dibutyrChemicalbookyl-cAMP hoʻi e kāohi i ka TNFalpha-induced rod cell apoptosis ma ke kāohi ʻana i ka hoʻoponopono FADD.
I loko o nā haʻawina vivo: Ma ke ʻano ʻiole, ua hoʻohuli ʻo Bucladesine (600nM/mouse, ip) i ka zinc chloride a me ke alakaʻi acetate i hoʻoulu i ka hoʻopaʻa ʻana i ka hoʻomanaʻo.
